By P.G. LeFevre
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Extra info for Active Transport through Animal Cell Membranes
Mg. P are adjusted to standard initial injection 5 . 10' counts/min. per kg. of body weight. (Courtesy of J. SACKS and Cold Spring Harbor Symposia) largely on the basis simply of the known physical and chemical make-up of the erythrocyte surface and consideration of the possible interplay of the ingredients by reason of their general chemical properties. Evidence of a more direct nature will of course be needed to establish these suggestions as a sound interpretation of the mechanics of the phosphate uptake.
Then placed at 25'C. in acetate medium; KCl added, to 10mM level, at zero time, after 10 min. of equilibration. Helow: Elfect of acetate and temperature on the process. K-level~ plotted are 30-minute levels, in experiments like those of graph above. (Courtesy of G. H. MUDGE and American Journal ot Physiology) 19 Phenolsulfonphthalein and p-aminohippurate, substances actively secreted by the kidney tubules and commonly used i~ kidney function tests. 33 VIII, 7, a: P. G. LEFEVRE, Active Transport through Animal Cell Membranes This same system has also been tackled by the Sheffield group; WHITTAM and DAVIES (1953 a, c) noted in these slices that the Na+ content rose rapidly, replacing the lost K +, if the metabolism was suppressed by cold or by lack of oxygen (Table VIII).
FOULKS et al. (1952) showed that under the influence of cortisone or testosterone, dogs excrete a large additional amount of thiosulfate, and that the drug carinamide (which depresses secretion of P AH, PSP and other organic anions) also reduces thiosulfate excretion to the point that its reabsorption becomes apparent. But a number of other inhibitors do not affect the system, nor does simultaneous handling of P AH. Thus the two sets of anion transport systems are evidently not identical. Intestinal Absorption of Ions It was established many years ago that salt solutions in the mammalian intestine are rather rapidly brought to a total tonicity not far from that of the blood; water and the chief inorganic ions migrate in the appropriate 4* 52 VIII, 7, a: P.
Active Transport through Animal Cell Membranes by P.G. LeFevre