Download e-book for iPad: Affinity Chromatography. Biospecific Sorption–The First by O. Hoffmann-Ostenhof

By O. Hoffmann-Ostenhof

ISBN-10: 0080226329

ISBN-13: 9780080226323

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By O. Hoffmann-Ostenhof

ISBN-10: 0080226329

ISBN-13: 9780080226323

Show description

Read or Download Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry PDF

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Extra resources for Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry

Sample text

Lowe with inert protein in the void v o l u m e . In c o n t r a s t , t h e s e enzymes displayed appreciable affinity for the parent i m m o b i l i s e d - A M P adsorbent (Tables 1 and 2) and could be eluted quantitatively with an NADH gradient as e x p e c t e d . On the other hand, IMP d e h y d r o g e n a s e f r o m E . coli binds tightly to I M P - S e p h a r o s e and r e q u i r e s either a p u l s e of 10 mM A M P or a l i n e a r gradient of A M P to effect elution ( F i g . 4 ) .

25). T h e s e o b s e r v a t i o n s s u g g e s t a s e c o n d m o r e p l a u s i b l e e x p l a n a t i o n for t h e d e c r e a s e d binding of e n z y m e s t o l i g a n d s a t t a c h e d to S e p h a r o s e v i a h y d r o p h i l i c s p a c e r m o l e c u l e s . T h e m o r e h y d r o p h i l i c d e r i v a t i v e s of A M P m a y h y d r o g e n bond to t h e p o l y s a c c h a r i d e b a c k ­ bone and t h e r e f o r e m a y not be p h y s i c a l l y a v a i l a b l e f o r i n t e r a c t i o n with t h e i r c o m p l e m e n t a r y e n z y m e s .

72, 669 (1975). (12) A . R u i z - C a r r i l l o & V . G . A u f r e y , A m e t h o d for t h e p u r i f i c a t i o n of h i s t o n e f r a c t i o n F 3 by affinity c h r o m a t o g r a p h y , A r c h s . B i o c h e m . B i o p h y s . 154, 185 (1973). (13) D . B . C r a v e n , M . J . H a r v e y , C . R . Lowe & P . D . G . D e a n , Affinity c h r o m a t o g r a p h y on i m m o b i l i s e d a d e n o s i n e 5 ' - m o n o p h o s p h a t e 1. A new s y n t h e s i s and s o m e p r o p e r t i e s of an N - i m m o b i l i s e d 5 ' - A M P , E u r .

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Affinity Chromatography. Biospecific Sorption–The First Extensive Compendium on Affinity Chromatography as Applied to Biochemistry and Immunochemistry by O. Hoffmann-Ostenhof


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